Titlebook: Split Inteins; Methods and Protocol Henning D. Mootz Book 2017 Springer Science+Business Media New York 2017 split-intein fusion proteins.m

出租

书目名称Split Inteins影响因子(影响力)




书目名称Split Inteins影响因子(影响力)学科排名




书目名称Split Inteins网络公开度




书目名称Split Inteins网络公开度学科排名




书目名称Split Inteins被引频次




书目名称Split Inteins被引频次学科排名




书目名称Split Inteins年度引用




书目名称Split Inteins年度引用学科排名




书目名称Split Inteins读者反馈




书目名称Split Inteins读者反馈学科排名

话

1064-3745 ation advice from the experts.This volume focuses on applications of split inteins, and the progress that has been made in the past 5 years on discovery and engineering of fast and more efficient split inteins. The first few chapters in .Split Inteins: Methods and Protocols. explore new techniques o

fastness

Intracellular Production of Cyclic Peptide Libraries with SICLOPPS,ences. SICLOPPS allows the ring size, set residues and number of random residues within a library to be predetermined by the user. SICLOPPS libraries have been combined with a variety of cell-based screens to identify cyclic peptide inhibitors of a variety of enzymes and protein–protein interactions.

歪曲道理

Photocontrol of the Src Kinase in Mammalian Cells with a Photocaged Intein, details on experimental design and the utilization of this photocaged intein to photoactivate the Src tyrosine kinase in human embryonic kidney (HEK) 293T cells. The described procedures may be adopted to photocontrol other proteins in other types of mammalian cells.

Narcissist

A Cassette Approach for the Identification of Intein Insertion Sites,s issues can arise, ranging from reduced stability to impairment of protein folding. In this chapter I address how the usage of DNA encoded intein cassettes can streamline and speed up the identification of functional split intein insertion sites in novel proteins of interest (POI).

MELON

Computational Prediction of New Intein Split Sites,new terminal sites are promising candidates for the generation of new split sites with the backbone opening being tolerated by the structural scaffold. Here we show how to integrate the online usage of the CP predictor, CPred, in the search of new split intein sites.

Missile

Purification of Microbially Expressed Recombinant Proteins via a Dual ELP Split Intein System,e the purification procedure can take place. Here we demonstrate a split-intein method, where the self-cleaving intein is divided into two inactive segments during expression and purification. Spontaneous assembly of the purified intein segments then restores self-cleaving activity to deliver the tagless target protein.

行业

薄荷醇

Ribosomal Synthesis of Thioether-Bridged Bicyclic Peptides,reactive amino acid. This approach can be applied to direct the formation of structurally diverse bicyclic peptides with high efficiency and selectivity in living . cells and provides a platform for the generation of combinatorial libraries of genetically encoded bicyclic peptides for screening purposes.

COMMA

Preparation of Semisynthetic Peptide Macrocycles Using Split Inteins,ynthetic macrocycles that would benefit from both the advantages associated with synthetic and genetically encoded parts. We describe in this chapter a chemo-enzymatic method to make semisynthetic cyclic peptides in vitro from two fragments using protein .-splicing and bioorthogonal oxime ligation.