Titlebook: Split Inteins; Methods and Protocol Henning D. Mootz Book 2017 Springer Science+Business Media New York 2017 split-intein fusion proteins.m

协定

越自我

Generic-Drug

Segmental Isotope Labeling of Insoluble Proteins for Solid-State NMR by Protein Trans-Splicing,usly difficult to study due to their insoluble and non-crystalline nature. However, assignment ambiguities because of limited resolution and spectral crowding are currently major hurdles that quickly increase with the length of the polypeptide chain. The line widths of ssNMR signals are independent

Measured

喧闹

A Recessive Pollination Control System for Wheat Based on Intein-Mediated Protein Splicing,scribed. The N- and C-terminal fragments of a barnase gene from . were fused to intein sequences from . sp. and transformed into wheat plants. Upon translation, both barnase fragments are assembled by an autocatalytic intein-mediated .-splicing reaction, thus forming a cytotoxic enzyme. This chapter

Amenable

Conditional Toxin Splicing Using a Split Intein System,on of a potent protein toxin and tethering each toxin fragment to a split intein fragment. The toxin–intein fragments are, in turn, fused to dimerization domains, such that addition of a dimerizing agent reconstitutes the split intein. These chimeric toxin–intein fusions remain nontoxic until the di

FLIRT

词汇记忆方法

ureter

借喻

Computational Prediction of New Intein Split Sites,sign of new split inteins. A computational circular permutation (CP) prediction method facilitates the search for internal permissive sites to create artificial circular permutants. In this procedure, the original amino- and carboxyl-termini are connected and new termini are created. The identified