Titlebook: Split Inteins; Methods and Protocol Henning D. Mootz Book 2017 Springer Science+Business Media New York 2017 split-intein fusion proteins.m

留恋

s and explicit illustrative figures.Treats complex function This book serves as a concise textbook for students in an advanced undergraduate or first-year graduate course in various disciplines such as applied mathematics, control, and engineering, who want to understand the modern standard of numer

使腐烂

Affinity Purification of Proteins in Tag-Free Form: Split Intein-Mediated Ultrarapid Purification ( cleavage reaction causes tagless POI to be released into the flow-through. The intein cleavage reaction is strongly inhibited by divalent ions (e.g., Zn.) under non-reducing conditions and is significantly enhanced by reducing conditions. The POI is cleaved efficiently regardless of the identity of

捏造

ZEST

Protein Chemical Modification Inside Living Cells Using Split Inteins, using several human cell lines. Moreover, we have shown that this approach can be also used for modifying proteins in order to control their cellular localization and potentially alter their biological activity.

Employee

Segmental Isotopic Labeling of Proteins for NMR Study Using Intein Technology,u Rev Biochem 72:249–289, 2003) and protein .-splicing (Shah et al., J Am Chem Soc 134:11338–11341, 2012). Here, we describe general implementation and latest improvements of expressed protein ligation method for the production of segmental isotopic labeled NMR samples.

漂亮才会豪华

使闭塞

A Recessive Pollination Control System for Wheat Based on Intein-Mediated Protein Splicing,ants. The use of this principle for the production of hybrid wheat seed is described. The suggested toolbox will hopefully be a valuable contribution to future optimization strategies in this commercially important crop.

Pde5-Inhibitors

abysmal

unctions. In many practical cases, however, such a solution is quite difficult or unavailable, and numerical approximations are called for. Modern development of computers accelerates the situation and, moreove978-981-19-9262-9978-981-19-9263-6Series ISSN 2038-5714 Series E-ISSN 2532-3318

正常

1064-3745 sette-based approach to quickly test many intein insertion positions, and a computational approach to predict new intein split sites (the approach also works for other proteins). 978-1-4939-8204-2978-1-4939-6451-2Series ISSN 1064-3745 Series E-ISSN 1940-6029