Titlebook: Split Inteins; Methods and Protocol Henning D. Mootz Book 2017 Springer Science+Business Media New York 2017 split-intein fusion proteins.m

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Split-Intein Triggered Protein Hydrogels,on, these hydrogels are able to undergo rapid reassembly after shear-induced rupture. Incorporation of an appropriate binding motif into the protein block copolymers enables the convenient site-specific incorporation of functional globular proteins into the hydrogel network.

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Conditional Toxin Splicing Using a Split Intein System,sensitivity. The following method outlines design criteria and implementation notes for CTS using a previously engineered system for splicing a toxin called sarcin, as well as for developing alternative CTS systems.

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Affinity Purification of Proteins in Tag-Free Form: Split Intein-Mediated Ultrarapid Purification (, needed for many applications, suffer from poor efficiency and/or high cost. Here we describe a simple, efficient, and potentially low-cost approach—split intein-mediated ultrarapid purification (SIRP)—for both the purification of the desired tagged protein from . lysate and removal of the tag in l

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Recombinant Expression of Cyclotides Using Split Inteins,opology of three disulfide bridges, with one disulfide penetrating through a macrocycle formed by the two other disulfides and inter-connecting peptide backbones, forming what is called a cystine knot topology. Naturally occurring cyclotides have shown to posses various pharmacologically relevant ac

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Ribosomal Synthesis of Thioether-Bridged Bicyclic Peptides,ramolecular linkage connecting two side chains of the peptide. Accordingly, methods to access macrocyclic peptides sharing this overall topology could be of significant value toward the discovery of new functional entities and bioactive compounds. With this goal in mind, we recently developed a stra

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Preparation of Semisynthetic Peptide Macrocycles Using Split Inteins,otentially superior physicochemical properties as compared to their linear counterparts, they are considered as ideal candidates for studying protein–protein interactions, among others. Most of the methods developed in recent years to prepare cyclic peptides focus either on a synthetic or a recombin

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Semisynthesis of Membrane-Attached Proteins Using Split Inteins, the biophysical properties of proteins as well as to study certain proteins in their native environment. Here, we describe the use of split inteins for the C-terminal attachment of lipid-modified peptides to virtually any protein of interest (POI) via protein .-splicing (PTS). To achieve this, the