Titlebook: Ribosome Biogenesis; Methods and Protocol Karl-Dieter Entian Book‘‘‘‘‘‘‘‘ 2022 The Editor(s) (if applicable) and The Author(s) 2022 cryo-el

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Fabian Teubl,Katrin Schwank,Uli Ohmayer,Joachim Griesenbeck,Herbert Tschochner,Philipp Milkereit

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A Comparative Perspective on Ribosome Biogenesis: Unity and Diversity Across the Tree of Lifeing) what is the molecular basis; (d) considering the universal ribosome conservation, what is the capability of functional plasticity and cellular adaptation of the ribosome biogenesis pathway?.In this review, we provide a brief overview of ribosome biogenesis across the tree of life and try to ill

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Chemical Modifications of Ribosomal RNA in yeast are highly conserved in eukaryotes including humans. Mutations in factors involved in rRNA modification are linked to several rare severe human diseases (e.g., X-linked Dyskeratosis congenita, the Bowen–Conradi syndrome and the William–Beuren disease). In this chapter, we summarize all rRN

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Non-radioactive In Vivo Labeling of RNA with 4-Thiouraciland high-throughput analysis, have greatly expanded our understanding of RNA metabolism across various cell types, organisms, and growth conditions..In this chapter, we describe a step-by-step protocol allowing pulse labeling of RNA with the nonradioactive nucleotide analog, 4-thiouracil, in the euk

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Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/830171.jpg

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https://doi.org/10.1007/978-1-0716-2501-9cryo-electron microscopy; ribosome biogenesis; subtunit protein composition; eukaryotes; maturation; Open

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