Titlebook: Ribosome Biogenesis; Methods and Protocol Karl-Dieter Entian Book‘‘‘‘‘‘‘‘ 2022 The Editor(s) (if applicable) and The Author(s) 2022 cryo-el

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Specialization of RNA Polymerase I in Comparison to Other Nuclear RNA Polymerases of ,highly specialized RNAPs to transcribe the nuclear genome. RNAP I synthesizes almost exclusively ribosomal (r)RNA, RNAP II synthesizes mRNA as well as many noncoding RNAs involved in RNA processing or RNA silencing pathways and RNAP III synthesizes mainly tRNA and 5S rRNA. This review discusses func

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Chemical Modifications of Ribosomal RNArtoire of RNAs, and fine-tune their functions. Ribosomal RNA in yeast contains more than 100 chemically modified residues in the functionally crucial and evolutionary conserved regions. The chemical modifications in the rRNA are of three types—methylation of the ribose sugars at the C2-positionAbstr

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In Vitro Selection of Deoxyribozymes for the Detection of RNA Modificationsfor detection of modifications in target RNAs and provide an alternative to traditional and modern methods for detection of ribose or nucleobase methylation. However, there are only few examples of DNA enzymes that specifically reveal the presence of a certain type of modification, including ..-meth

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Non-radioactive In Vivo Labeling of RNA with 4-Thiouraciltion to environmental changes. Accordingly, analyzing RNA expression remains an important step to understand the molecular basis controlling the formation of cellular phenotypes, cellular homeostasis or disease progression. Steady-state RNA levels in the cells are controlled by the sum of highly dyn