Titlebook: Ribosome Biogenesis; Methods and Protocol Karl-Dieter Entian Book‘‘‘‘‘‘‘‘ 2022 The Editor(s) (if applicable) and The Author(s) 2022 cryo-el

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Ribosome Biogenesis978-1-0716-2501-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Preparation of RNA Polymerase Complexes for Their Analysis by Single-Particle Cryo-Electron Microsco carbon support films. In sum, we present an efficient workflow for cryo-grid preparation and optimization, including early stage cryo-EM screening that can be adapted to a wide range of soluble samples for high-resolution structure determination.

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In Vitro Selection of Deoxyribozymes for the Detection of RNA Modificationsed RNA substrates. This protocol describes a general in vitro selection stagtegy to evolve new RNA-cleaving DNA enzymes that can efficiently differentiate modified RNA substrates from their unmodified counterpart.

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Mapping of the Chemical Modifications of rRNAser RNA molecules. The chemical nature, their precise location and quantification of modifications will facilitate understanding the precise role of these chemical modifications in cellular physiology.

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A Comparative Perspective on Ribosome Biogenesis: Unity and Diversity Across the Tree of Liferoteins. Accordingly, ribosome biogenesis is a fundamental cellular process required for functional ribosome homeostasis and to preserve satisfactory gene expression capability..Although the ribosome is universally conserved, its biogenesis shows an intriguing degree of variability across the tree o

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Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryoteshis high output is achieved by the presence of multiple copies of rRNA genes in eukaryotic genomes transcribed at a high rate. In contrast to most of the other transcribed genomic loci, actively transcribed rRNA genes are largely devoid of nucleosomes adapting a characteristic “open” chromatin state