Titlebook: RNA Remodeling Proteins; Methods and Protocol Marc Boudvillain Book 2015 Springer Science+Business Media New York 2015 DEAD-box Helicase.EM

出来

Recombineering Applications for the Mutational Analysis of Bacterial RNA-Binding Proteins and Theirb neighboring sequences. Methods for performing such “markerless” site-directed chromosomal mutagenesis in bacteria have been developed in recent years. One such technique, used routinely in our laboratory, is described here.

卵石

A FRET-Based, Continuous Assay for the Helicase Activity of DEAD-Box Proteins,ion of duplexes and isolated strands. Here, we describe a continuous, FRET-based assay that allows the measurement of the helicase activities of DEAD-box proteins with a precision high enough to detect changes in activation enthalpies associated with cold adaptation.

织物

Constructing a Magnetic Tweezers to Monitor RNA Translocation at the Single-Molecule Level,nstruction of a simple magnetic trap and, in particular, on the detailed description of image analysis methods to measure the extension changes in nucleic-acid molecules induced by protein activity. Finally, we carefully describe the steps involved in performing a full magnetic tweezers experiment.

耐寒

Monitoring RNA Unwinding by the Transcription Termination Factor Rho from ,, preparation and in vitro characterization (ATPase and helicase activities) of the Rho factor from ., a specimen with uncharacteristic molecular and enzymatic features. These methods set the stage for future studies aimed at better defining the diversity of enzymatic properties of Rho across the bacterial kingdom.

ARBOR

香料

Sinus-Rhythm

Key Points to Consider When Studying RNA Remodeling by Proteins,roteins that function as chaperones and remodelers. We then use these properties as a foundation to explore key points for the design and interpretation of experiments that probe RNA rearrangements and their acceleration by proteins.

带来墨水

Biochemical Characterization of G4 Quadruplex Telomerase RNA Unwinding by the RNA Helicase RHAU,bination of total RNA and quadruplex-specific dyes to observe the expected quadruplex to duplex conversion. This straightforward method can be extended to study structural changes in other inter- or intramolecular quadruplex containing DNA/RNA molecules with the RHAU helicase or other RNA/DNA remodeling enzymes.

abstemious

Mechanisms of HCV NS3 Helicase Monitored by Optical Tweezers,resolution optical tweezers study of NS3 helicase, starting from the purification of the recombinant helicase protein from . to setting up a high-resolution single-molecule experiment using optical tweezers.

omnibus