Titlebook: RNA Remodeling Proteins; Methods and Protocol Marc Boudvillain Book 2015 Springer Science+Business Media New York 2015 DEAD-box Helicase.EM

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Bioinformatics and Biochemical Methods to Study the Structural and Functional Elements of DEAD-Box binding ATP and RNA, and that are needed for the enzymatic activities. The conserved sequence motifs and structural homology indicate that these proteins share common origins and underlining functionality. Indeed, the purified proteins generally act as ATP-dependent RNA-binding proteins and RNA-dep

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Measuring Helicase Inhibition of the DEAD-Box Protein Dbp2 by Yra1,ines DEAD-box protein specificity is enzymatic regulation by other protein cofactors. In this chapter, we describe a protocol for purifying the . DEAD-box RNA helicase Dbp2 and RNA-binding protein Yra1 and subsequent analysis of helicase regulation. The experiments described here can be adapted to o

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Monitoring RNA Unwinding by the Transcription Termination Factor Rho from ,,iptional RNA–DNA duplexes (R-loops) in bacteria. The molecular mechanisms underlying these biological functions have been essentially studied with Rho enzymes from . or close Gram-negative relatives. However, phylo-divergent Rho factors may have distinct properties. Here, we describe methods for the

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Characterization of the Mechanisms of Transcription Termination by the Helicase Sen1,pled processes. Here we describe an in vitro transcription-termination assay that we have used for the analysis of the mechanism of termination by the yeast helicase Sen1. In this system, we use highly purified proteins to assemble ternary elongation complexes (RNA polymerase, DNA template, and nasc

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