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Titlebook: RNA Remodeling Proteins; Methods and Protocol Marc Boudvillain Book 2015 Springer Science+Business Media New York 2015 DEAD-box Helicase.EM

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书目名称RNA Remodeling Proteins
副标题Methods and Protocol
编辑Marc Boudvillain
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproductible results.Contains key notes and implementation advice from the experts.Includes supplementary materi
丛书名称Methods in Molecular Biology
图书封面Titlebook: RNA Remodeling Proteins; Methods and Protocol Marc Boudvillain Book 2015 Springer Science+Business Media New York 2015 DEAD-box Helicase.EM
描述.This volume. .provides readers with current methods to study RNA remodeling proteins. The methods, ranging from basic to complex, help the scientific community understand the role and fate of RNA species in cells, and their structures and interactions with other biomolecules. The book begins with two introductory chapters, followed by chapters where readers will find procedures to identify RNA remodeling proteins and their cofactors, physiological RNA targets and biological functions, and complex molecular mechanisms of action using purified components. Written in the highly successful .Methods of Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .RNA Remodeling Proteins: Methods and Protocols. seeks to aid scientists in the further study of this ever evolving field of proteins and mechanisms..
出版日期Book 2015
关键词DEAD-box Helicase; EMOTE; Fluorescence Resonance Energy Transfer (FRET); RHAU; RNA Helicase; RNA targets;
版次1
doihttps://doi.org/10.1007/978-1-4939-2214-7
isbn_softcover978-1-4939-4899-4
isbn_ebook978-1-4939-2214-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
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Dynamics of the Spb4 Interactome Monitored by Affinity Purification,s has been established as one of the most useful techniques to explore the yeast ribosome biogenesis pathway. In this chapter, we describe the use of different affinity purification methods to study the physical environment of RNA helicases involved in ribosome biogenesis, using as an example the pu
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Cellular Localization of RNA Degradation and Processing Components in ,,ion of labeled proteins. In addition, when antibodies against native proteins are not available or lack specificity, epitope tags such as hemagglutinin (HA) or Flag can be used to label chromosomally expressed proteins. The short Flag- and HA-tag, eight or nine amino acids, are unlikely to interfere
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