Titlebook: RNA Remodeling Proteins; Methods and Protocol Marc Boudvillain Book 2015 Springer Science+Business Media New York 2015 DEAD-box Helicase.EM

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Key Points to Consider When Studying RNA Remodeling by Proteins,dence arises from intrinsic properties of RNA structure. Specifically, RNAs possess stable local structure, largely in the form of helices, and they have abundant opportunities to form alternative helices and tertiary contacts and therefore to populate alternative structures. Proteins with RNA chape

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In Vivo Cross-Linking Followed by PolyA Enrichment to Identify Yeast mRNA Binding Proteins, understand this process, it is necessary to identify the complete set of mRNA binding proteins. This work describes a method for the systematic identification of yeast mRNA binding proteins. This method applies in vivo UV cross-linking, affinity pull-down of polyA(+) mRNAs, and analysis by mass spe

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Dynamics of the Spb4 Interactome Monitored by Affinity Purification,ukaryotic model organism .. In yeast, genetic and biochemical analyses indicate that these RNA helicases are energy-consuming modulators of local structures inside pre-ribosomal particles that actively promote the establishment or dissociation of snoRNA:pre-rRNA base pairings, the activity of certai

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,Using EMOTE to Map the Exact 5′-Ends of Processed RNA on a Transcriptome-Wide Scale,oswitches, but it is also the case for many mRNAs, where secondary structures in the 5′ or 3′ UTR can determine the efficiency of translation or the half-life of the RNA. There are paths to modify such secondary structures, (1) by the action of a helicase that allows an alternative RNA structure to

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Determination of RNA Chaperone Activity Using an , Mutant,splays a cold-sensitive growth phenotype. Plant cold shock domain (CSD) proteins have been shown to complement the cold-sensitive phenotype of the . mutant and to share a function with . CSPs as RNA chaperones. This methodology, which is detailed here, can be utilized to reveal or probe the RNA chap

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