Titlebook: In Vitro Differentiation of T-Cells; Methods and Protocol Shin Kaneko Book 2019 Springer Science+Business Media, LLC, part of Springer Natu

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,Redifferentiation of Adaptive Naïve-Like CTL from T-Cell-Derived iPSC, capacity while retaining equivalent effector function compared to parental T-cell clones. Here, we demonstrate the methodology to produce naïve-like T-iPSC-Ts, which could be potent cell source for adoptive immunotherapy.

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Flow Cytometry Analysis to Identify Human CD8+ T Cells,ity, cell components (DNA, mRNA), surface receptors, intracellular proteins, and signaling events. The flow cytometer operates via three main systems: the fluidics, optics, and electronics, which work together to analyze the physical and chemical properties of your sample. The first system, the flui

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Flow Cytometry Analysis to Identify Human CD4+ T Cell Subsets,he expression of cell surface markers and intracellular molecules to define cells into different populations using cell size, granularity, and fluorescently labeled antibodies. Thus, flow cytometry enables simultaneous and mutliparameter analysis of single cells..During the staining procedure, a sin

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Gene Modification and Immunological Analyses for the Development of Immunotherapy Utilizing T Cells antigen-specific antitumor receptors, such as T-cell receptor (TCR) and chimeric antigen receptor (CAR), has demonstrated clinical benefits even in patients with refractory malignancies. To advance this treatment modality, both generation of gene-modified T cells and evaluation of their reactivity

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In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells,cells from patients may effectively destroy the original tumor cells. One of the limitations is a rapid acquisition of tolerant (anergy, deletion, dysfunctional, and/or exhausted) phenotypes. We and others found that stem cell memory T (T.) cells are strongly resistant to tolerance, showing strong e

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