Titlebook: In Vitro Differentiation of T-Cells; Methods and Protocol Shin Kaneko Book 2019 Springer Science+Business Media, LLC, part of Springer Natu

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In Vitro Differentiation of T Cells: From Human Embryonic Stem Cells and Induced Pluripotent Stem C) and induced pluripotent stem cells (iPSCs) derived from non-T cells, followed by the differentiation of the T-cell lineage. Derivation of T cells from PSCs involves three steps: induction of PSCs to hematopoietic progenitor cells (HPCs), differentiation of HPCs into progenitor T cells, and maturat

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,Redifferentiation of Adaptive Naïve-Like CTL from T-Cell-Derived iPSC,re is derived from T-cell clone (T-iPSC), which has lost naïve phenotype and acquired exhaustion/senescence phenotype during cloning process (.). On the other hand, redifferentiated T cells (T-iPSC-Ts) reacquire naïve phenotype (CD45RA.CD45RO.CCR7.CD62L.), which are reportedly critical for in vivo p

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In Vitro Differentiation of T Cell: From Human iPS Cells in Feeder-Free Condition,cribe a feeder-free culture condition for differentiating T cells from hematopoietic cells that are cultured on Fc-DLL4-coated plate with T-lineage cytokines. This condition is capable of efficiently differentiating hematopoietic progenitor cells (HPCs) to immature T cells expressing both CD4 and CD

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In Vitro Differentiation of T Cell: From CAR-Modified T-iPSC,al malignancies. Allogeneic transplantation approach is promising for broaden application of CART therapy. iPSCs are one of the ideal cell sources for this approach. CAR-engineered iPSCs are demonstrated to give rise to CAR-engineered T cell and exert their effector function. In this section, we des

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In Vitro Detection of Cellular Adjuvant Properties of Human Invariant Natural Killer T Cells, by their specific ligand α-galactosylceramide (α-GalCer) induces the activation of dendritic cells (DCs) via reciprocal interaction, which results in the generation of cellular immunity against cancer. Here we describe a method to detect DC-mediated cellular adjuvant properties of human iNKT cells

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