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Titlebook: Immunogenetics; Methods and Applicat Frank T. Christiansen,Brian D. Tait Book 2012 Springer Science+Business Media New York 2012 DNA sequen

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Impact of HLA Matching and HLA Antibodies in Organ Transplantation: A Collaborative Transplant Studomplex problems and risks involved in human organ transplantation. Currently, more than 400 transplant centers in 45 countries are contributing to this voluntary international effort, and from the beginning of the study, the impact on graft outcome of immunological factors, such as matching for HLA
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HLA Antibody Detection and Characterization by Solid Phase Immunoassays: Methods and Pitfalls,e and reagent efficiency, compared to the traditionally used cell-based methods. Testing is performed using commercially available test kits. The assays are of two general types: enzyme-linked immunosorbent assays and multianalyte bead. The types vary in both sensitivity and equipment requirements..
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Detection and Characterisation of Alloreactive T Cells,unoassays for ex vivo monitoring and quantitation of alloreactive T cells are being increasingly utilised to provide valuable information for individualised clinical management of transplant recipients. Here we describe detailed methodologies for both traditional and novel assays utilised for the de
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Detection of Allo-HLA Cross-Reactivity by Virus-specific Memory T-Cell Clones Using Single HLA-Transplantation field. Viral peptide/HLA tetrameric complexes allow the rapid generation of virus-specific clones using single cell sorting apparatus, permitting the determination of alloreactivity from a single TCR with known specificity. When combined with new target “detector” cells called single HLA
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