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Titlebook: Immunogenetics; Methods and Applicat Frank T. Christiansen,Brian D. Tait Book 2012 Springer Science+Business Media New York 2012 DNA sequen

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Methods for Diagnostic HLA Typing in Disease Association and Drug Hypersensitivity,utilise a combination of polymerase chain reaction (PCR) amplification to detect sequence polymorphism by the presence or absence of amplification, nucleotide sequencing of the PCR product, and hybridisation of the PCR product with labelled probes. The probes are specific for sequence polymorphism a
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HLA Typing Using Bead-Based Methods,igens (HLAs). The principle of these assays involves the hybridization of reverse sequence-specific oligonucleotide probes each attached to a unique colour coded microsphere to identify HLA class I and class II alleles. Target DNA is polymerase chain reaction (PCR) amplified using group-specific pri
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Molecular Analysis of Complement Component C4 Gene Copy Number,the prevention of immune complex precipitation and in clearance of immune complexes..Two isotypes of C4—C4A and C4B—are coded by genes located at two loci within the major histocompatibility complex (MHC) on chromosome 6. While these isotypes share over 99% amino acid sequence homology, five nucleot
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High Resolution MICA Genotyping by Sequence-Based Typing (SBT),orphisms and distinguishes the GCT repeats in exon 5. One PCR amplification was performed to obtain templates of 2.2 kb, including exons 2, 3, 4, and 5 of MICA to be sequenced with two forward and two reverse primers. Overlay of nucleotide sequencing signals resulting from presence of different GCT
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