Titlebook: G-Quadruplex Nucleic Acids; Methods and Protocol Danzhou Yang,Clement Lin Book 2019 Springer Science+Business Media, LLC, part of Springer

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G-Quadruplex DNA and RNA,-rich DNA and RNA sequences, G-quadruplexes can readily form under physiologically relevant conditions and are globularly folded structures. DNA is widely recognized as a double-helical structure essential in genetic information storage. However, only ~3% of the human genome is expressed in protein;

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Revealing the Energetics of Ligand-Quadruplex Interactions Using Isothermal Titration Calorimetry,ptimization of potent G-quadruplex ligands for use in therapeutics, diagnostics, or other technological applications. Isothermal titration calorimetry (ITC) can resolve energetic contributions to complex formation and constitutes the only available experimental method to directly measure binding ent

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Biosensor-Surface Plasmon Resonance: Label-Free Method for Investigation of Small Molecule-Quadruplreal time, without the need for labeling any components of the interaction. The method provides real-time equilibrium and kinetic characterization for quadruplex DNA interactions and requires small amounts of materials and no external probe. A detailed protocol for quadruplex-DNA interaction analyse

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Pericarditis

G-Quadruplex Stability from DSC Measurements,cterized thermodynamically, providing the information on the stability of various G4 conformations. We show how the relevant thermodynamic and sometimes kinetic parameters are obtained by employing differential scanning calorimetry (DSC) and global fitting of an appropriate model to the DSC data. We

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X-Ray Crystallographic Studies of G-Quadruplex Structures, researchers with exciting opportunities to explore new structural arrangements of putative G4 forming sequences and investigate their recognition by small molecule compounds. The crowded and ordered crystalline environment requires the self-assembly of stable G4 motifs, allowing for an understandin

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NMR Studies of G-Quadruplex Structures and G-Quadruplex-Interactive Compounds,quadruplex structures have been found to form in nucleic acid regions of biological significance, including human telomeres, gene promoters, and untranslated regions of mRNA. Thus, they are considered attractive therapeutic targets. Nuclear magnetic resonance (NMR) spectroscopy is a powerful method

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Using Molecular Dynamics Free Energy Simulation to Compute Binding Affinities of DNA G-Quadruplex LNA. Such calculations have a number of applications, such as rescoring docking results and validating docked poses, to inform the discovery of G-quadruplex binders with high affinity and selectivity. This chapter describes two binding free energy protocols: the double decoupling method (DDM) and the

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Electrophoretic Mobility Shift Assay and Dimethyl Sulfate Footprinting for Characterization of G-Quumulating evidence supporting G-quadruplex involvement in a number of important aspects of genome functions, including transcription, replication, and genomic stability, and that protein and enzyme recognition of G-quadruplexes may represent a key event to regulate physiological or pathological path