Titlebook: Developmental Biology of the Sea Urchin and Other Marine Invertebrates; Methods and Protocol David J. Carroll,Stephen A. Stricker Book 2014

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Multicolor Labeling in Developmental Gene Regulatory Network Analysis,lor fluorescent in situ hybridization (FISH) as well as a combination of FISH and immunohistochemistry in sea urchin embryonic GRN studies. The methods presented here can be applied to a variety of experimental settings where accurate spatial resolution of multiple gene products is required for cons

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Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/270071.jpg

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https://doi.org/10.1007/978-3-476-99751-7e (>1 mm) veliger larvae of this species are well suited for neuroethological investigations of settlement and metamorphosis. In this chapter, methods are described for conditioning adult broodstock, growing microalgal food for larvae, and culturing larvae to metamorphic competence in artificial sea