Titlebook: Developmental Biology of the Sea Urchin and Other Marine Invertebrates; Methods and Protocol David J. Carroll,Stephen A. Stricker Book 2014

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Microinjection and 4D Fluorescence Imaging in the Eggs and Embryos of the Ascidian ,,ortical structures like the CAB in ascidians, etc.) combined with targeted gene knockdown during embryonic development is a powerful technique to understand the mechanisms of embryonic development. The eggs and embryos of the primitive marine chordate . are an excellent model system for combining li

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Biotinylation of Oocyte Cell Surface Proteins of the Starfish , ,rom the cell surface to relevant intracellular compartments. To identify starfish oocyte and egg cell surface proteins, a biotinylation method was adapted from prior protocols using B cells, leukocytes, mouse oocytes, and sea urchin eggs (Cole et al. Mol Immunol 24:699–705, 1987; Flaherty and Swann

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Immunoblotting Analyses of Changes in Protein Phosphorylations During Oocyte Maturation in Marine Nar extracts. This chapter describes a traditional, film-based immunoblotting method for monitoring the phosphorylation status of proteins in marine nemertean oocytes undergoing maturation. Similarly, with minor modifications, the protocol could potentially be applied to a wider variety of cellular p