Titlebook: Developmental Biology of the Sea Urchin and Other Marine Invertebrates; Methods and Protocol David J. Carroll,Stephen A. Stricker Book 2014

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吵闹

Rearing Gymnolaemate Bryozoan Larvae for Cellular and Molecular Analyses,o three types, cyphonautes (shelled, feeding), pseudocyphonautes (shelled, nonfeeding), and coronate (unshelled, nonfeeding), although each group is heterogeneous and probably includes various morphologies that are largely undescribed. Here, methods for rearing bryozoan colonies and larvae are presented.

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DAMN

GIST

Perturbations to the Hedgehog Pathway in Sea Urchin Embryos,hods to perturb the Hedgehog signaling pathway in the sea urchin. These include microinjection of Morpholinos and mRNA constructs as well as treatments with small molecule inhibitors. Finally we provide simple methods for assaying Hedgehog phenotypes in the sea urchin embryo.

GIST

Immunoblotting Analyses of Changes in Protein Phosphorylations During Oocyte Maturation in Marine Nmertean oocytes undergoing maturation. Similarly, with minor modifications, the protocol could potentially be applied to a wider variety of cellular processes and extract types that might be analyzed in other investigations of marine invertebrate development.

COMA

https://doi.org/10.1007/978-3-031-66108-2ve cell imaging with gene knockdown experiments. Here we describe simple methods for microinjecting . eggs with mRNA encoding fluorescent fusion proteins combined with 4D time-lapse imaging techniques we use to follow all of embryonic development from the egg to late tailbud stage.

Pandemic

Interest of Non-destructive Tests,escribe expression and purification of GFP/RFP-tagged proteins involved in regulation of DNA replication. We also explain the techniques used to introduce recombinant proteins and fluorescent tubulin into sea urchin eggs and embryos.

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