Titlebook: Developmental Biology of the Sea Urchin and Other Marine Invertebrates; Methods and Protocol David J. Carroll,Stephen A. Stricker Book 2014

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https://doi.org/10.1007/978-3-031-66108-2cue experiments. The simultaneous localization of an in situ RNA hybridization probe and a cell type specific antigen has enabled studies of gene expression in specific types of neurons. Fixatives and antibodies can be capricious; thus, we provide data on preservation of antigens with commonly used fixatives and buffers.

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Imaging Neural Development in Embryonic and Larval Sea Urchins,cue experiments. The simultaneous localization of an in situ RNA hybridization probe and a cell type specific antigen has enabled studies of gene expression in specific types of neurons. Fixatives and antibodies can be capricious; thus, we provide data on preservation of antigens with commonly used fixatives and buffers.

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1064-3745 otes and implementation advice from the experts.Includes sup.In .Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, .expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the la

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https://doi.org/10.1007/978-3-476-99751-7 are described for conditioning adult broodstock, growing microalgal food for larvae, and culturing larvae to metamorphic competence in artificial seawater. A protocol is also presented for obtaining electrophysiological recordings of ciliary arrest spikes from intact, behaving larvae, as putative neural correlates of larval settlement.

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https://doi.org/10.1007/978-3-476-99751-7o three types, cyphonautes (shelled, feeding), pseudocyphonautes (shelled, nonfeeding), and coronate (unshelled, nonfeeding), although each group is heterogeneous and probably includes various morphologies that are largely undescribed. Here, methods for rearing bryozoan colonies and larvae are presented.

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