Titlebook: Developmental Biology of the Sea Urchin and Other Marine Invertebrates; Methods and Protocol David J. Carroll,Stephen A. Stricker Book 2014

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Folgen sexualisierter Gewalt im BdP,hods to perturb the Hedgehog signaling pathway in the sea urchin. These include microinjection of Morpholinos and mRNA constructs as well as treatments with small molecule inhibitors. Finally we provide simple methods for assaying Hedgehog phenotypes in the sea urchin embryo.

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https://doi.org/10.1007/978-981-97-4414-5mertean oocytes undergoing maturation. Similarly, with minor modifications, the protocol could potentially be applied to a wider variety of cellular processes and extract types that might be analyzed in other investigations of marine invertebrate development.

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https://doi.org/10.1007/978-3-658-44702-1 exocytosis, cytoskeletal remodeling, and cell cycle reentry. Here we describe methods to investigate individual signaling proteins as well as global proteomic and phosphoproteomic changes involved in the initial steps of egg activation through the egg-to-embryo transition.

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BET

Isolation and Assessment of Signaling Proteins from Synchronized Cultures During Egg Activation and exocytosis, cytoskeletal remodeling, and cell cycle reentry. Here we describe methods to investigate individual signaling proteins as well as global proteomic and phosphoproteomic changes involved in the initial steps of egg activation through the egg-to-embryo transition.

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Isolating Specific Embryonic Cells of the Sea Urchin by FACS,, and conditions for FACS of a rare cell type in the embryo. This protocol may be adapted for analysis of mRNA, chromatin, protein, or carbohydrates and depends only on the probe availability for the cell of interest. We anticipate that this protocol will be broadly applicable to embryos of other sp

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