Titlebook: DNA Topoisomerase Protocols; Volume II: Enzymolog Neil Osheroff,Mary-Ann Bjornsti Book 2001 Humana Press 2001

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DNA-Unwinding Test Using Eukaryotic DNA Topoisomerase I, complete double-helical turn). The end result of such a nicking-rejoining process in the presence of a DNA-unwinding agent is the formation of a relaxed underwound DNA molecule with a deficient linking number (the number of times one strand goes around the other in a duplex ring). The relaxed under

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,Drug—DNA Interactions,y correlate with antitumor activity; however, modulation of topoisomerase . activity was shown to coincide with biological activity of these agents (.). Interactions of ligands with DNA are being explored using a variety of physical and biochemical methods in an effort to determine the chemical and

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Drug Toxicity in , Cells Expressing Human Topoisomerase I,single 5’-phosphodiester intermediate (.–.). The etopoI enzyme is very efficient at relaxing highly negatively supercoiled DNA and shows progressively decreasing activity as the substrate DNA becomes more relaxed (.,.). The htopoI enzyme relaxes both negatively and positively supercoiled DNA to comp

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Habiba Drias,Farouk Yalaoui,Allel HadjaliIn “inventing” reverse gyrase, the living world has built a unique and remarkably sophisticated enzyme that is more than a simple topoisomerase. Reverse gyrase possesses the unique ability to catalyze the production of positive supercoils in a closedcircular DNA at the expense of ATP (.); . . . for review.

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DNA Topoisomerase Protocols978-1-59259-057-5Series ISSN 1064-3745 Series E-ISSN 1940-6029

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