Titlebook: Cytochrome P450 Protocols; Ian R. Phillips,Elizabeth A. Shephard Book 2006Latest edition Humana Press 2006 Allele.Escherichia coli.Oxidati

Grating

MAL

Establishment of Functional Human Cytochrome P450 Monooxygenase Systems in ,,ent. This chapter provides a detailed technical description of the processes that allow the coexpression of various CYP isoforms together with CYP reductase in . and gives some examples of the results that can be achieved for the expression of human P450s.

和音

Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation,and an emission wavelength of 450 nm. This assay is applicable to enzymatic studies of cDNA-expressed CYP2A6 and can be used to monitor coumarin 7-hydroxylation activity in microsomes prepared from liver and other tissues and in isolated hepatocytes and cultured cells that express this cytochrome P450 activity.

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,CYP2D6-Dependent Bufuralol 1′-Hydroxylation Assayed by Reverse-Phase Ion-Pair High-Performance Liquonsisting of 30% acetonitrile and 2 m. perchloric acid, with detection by fluorescence using an excitation wavelength of 252 nm and an emission wavelength of 302 nm. This method is applicable to enzymatic studies for determination of CYP2D6-catalyzed bufuralol 1′-hydroxylation activity.

AXIS

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SOB

Hans Peter Latscha,Helmut Alfons Kleinsposition in . and site-specific recombination in vitro; adaptation of . (.) 9 cells to shaking culture and to serum-free medium; cryopreservation and transfection of .9 cells; amplification of baculovirus and determination of viral titer; analysis of baculoviral DNA; and expression and analysis of recombinant proteins.

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Hans Peter Latscha,Helmut Alfons Kleinrations are achieved using a C. column with a linear gradient of 10–100% methanol, with detection at 230 nm. This method is applicable to enzymatic studies for determination of CYP2C8-catalyzed paclitaxel 6α-hydroxylation activity.

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