Titlebook: Cytochrome P450 Protocols; Ian R. Phillips,Elizabeth A. Shephard Book 2006Latest edition Humana Press 2006 Allele.Escherichia coli.Oxidati

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cyanosis

Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation,mixture, the enzymatic product, 7-hydroxycoumarin, is recovered by a double-extraction procedure and assayed using an excitation wavelength of 370 nm and an emission wavelength of 450 nm. This assay is applicable to enzymatic studies of cDNA-expressed CYP2A6 and can be used to monitor coumarin 7-hyd

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Determination of CYP2B6 Component of 7-Ethoxy-4-Trifluoromethylcoumarin ,-Deethylation Activity in ide, and may be an important determinant of clinical responses to these agents. A spectrofluorometric method is described for the determination of CYP2B6-catalyzed 7-ethoxy-4-trifluoromethylcoumarin .-deethylation activity in human liver microsomes. The specificity of this method for CYP2B6 is incre

AMOR

,High-Performance Liquid Chromatography Analysis of CYP2C8-Catalyzed Paclitaxel 6α-Hydroxylation,ivated in human liver by a cytochrome P450 (P450)-catalyzed 6α-hydroxylation reaction. A reverse-phase, high-performance liquid chromatographic assay is described for the analysis of paclitaxel 6α-hydroxylation catalyzed by human liver microsomes or cDNA-expressed P450 enzyme CYP2C8. Analytical sepa

Congestion

,Determination of CYP2C9-Catalyzed Diclofenac 4′-Hydroxylation by High-Performance Liquid Chromatogr microsomes or cDNA-expressed CYP2C9. Analytical separation is achieved using a C. column developed with a gradient of 30% acetonitrile and 2 m. perchloric acid in water to 100% methanol, with detection at 280 nm. This method is applicable to enzymatic studies for determination of CYP2C9-catalyzed d

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,CYP2C19-Mediated (,)-Mephenytoin 4′-Hydroxylation Assayed by High-Performance Liquid Chromatography.C-labeled (.)-mephenytoin following incubation with cDNA-expressed CYP2C19 or human liver microsomes. Analytical separation is achieved using a C. column developed with a gradient from 10 to 100% methanol, with detection using a scintillation detector. This method is applicable to enzymatic studies

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