| 书目名称 | Cytochrome P450 Protocols | | 编辑 | Ian R. Phillips,Elizabeth A. Shephard | | 视频video | | | 概述 | Includes supplementary material: | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source. | | 出版日期 | Book 2006Latest edition | | 关键词 | Allele; Escherichia coli; Oxidation; Termination; enzymes; hybridization; metabolism; reproducible techniqu | | 版次 | 2 | | doi | https://doi.org/10.1385/1592599982 | | isbn_softcover | 978-1-61737-572-9 | | isbn_ebook | 978-1-59259-998-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2006 |
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发表于 2025-3-21 19:41:05
