Titlebook: Cytochrome P450 Protocols; Ian R. Phillips,Elizabeth A. Shephard Book 2006Latest edition Humana Press 2006 Allele.Escherichia coli.Oxidati

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Komplexverbindungen Bindungen in Komplexenonitor P450 (P450) activity in both hepatic and extrahepatic tissues. A spectrofluorometric method is described for determination of P450-catalyzed 7-ethoxycoumarin .-deethylation. Following acidification of the incubation mixture, the enzymatic product, 7-hydroxycoumarin, is recovered by a double-e

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Cytochrome P450 Protocols978-1-59259-998-1Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Hans Peter Latscha,Helmut Alfons Klein microsomes or cDNA-expressed CYP2C9. Analytical separation is achieved using a C. column developed with a gradient of 30% acetonitrile and 2 m. perchloric acid in water to 100% methanol, with detection at 280 nm. This method is applicable to enzymatic studies for determination of CYP2C9-catalyzed diclofenac 4′-hydroxylation activity.

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,Determination of CYP2C9-Catalyzed Diclofenac 4′-Hydroxylation by High-Performance Liquid Chromatogr microsomes or cDNA-expressed CYP2C9. Analytical separation is achieved using a C. column developed with a gradient of 30% acetonitrile and 2 m. perchloric acid in water to 100% methanol, with detection at 280 nm. This method is applicable to enzymatic studies for determination of CYP2C9-catalyzed diclofenac 4′-hydroxylation activity.

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Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System,bolizing enzymes toward a particular substrate. In this chapter we describe a baculovirus/insect cell system that we have used for the expression of human and mouse flavin-containing monooxygenases. Methods are described for the generation of recombinant baculoviral DNAs, via both site-specific tran