Titlebook: Cytochrome P450 Protocols; Ian R. Phillips,Elizabeth A. Shephard Book 2006Latest edition Humana Press 2006 Allele.Escherichia coli.Oxidati

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defendant

Hans Peter Latscha,Helmut Alfons Klein and play a major role in the metabolism of many foreign chemicals and endogenous substances. We describe a spectrofluorometric method for determining 7-ethoxyresorufin .-dealkylation catalyzed by CYP1A1, CYP1A2, and CYP1B1. The formation of the enzymatic product, resorufin, is monitored continuousl

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Hans Peter Latscha,Helmut Alfons Kleinmixture, the enzymatic product, 7-hydroxycoumarin, is recovered by a double-extraction procedure and assayed using an excitation wavelength of 370 nm and an emission wavelength of 450 nm. This assay is applicable to enzymatic studies of cDNA-expressed CYP2A6 and can be used to monitor coumarin 7-hyd

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Hans Peter Latscha,Helmut Alfons Kleinivated in human liver by a cytochrome P450 (P450)-catalyzed 6α-hydroxylation reaction. A reverse-phase, high-performance liquid chromatographic assay is described for the analysis of paclitaxel 6α-hydroxylation catalyzed by human liver microsomes or cDNA-expressed P450 enzyme CYP2C8. Analytical sepa

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Hans Peter Latscha,Helmut Alfons Kleinincubation of bufuralol with cDNA-expressed CYP2D6 or human liver microsomes. Analytical separation is achieved using a C. column and a mobile phase consisting of 30% acetonitrile and 2 m. perchloric acid, with detection by fluorescence using an excitation wavelength of 252 nm and an emission wavele

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Hans Peter Latscha,Helmut Alfons KleinCYP3A enzymes from various species, including human, metabolize testosterone by a 6β-hydroxylation reaction, which is unique to this P450 subfamily. A thin-layer chromatographic method is described for the determination of 6β-hydroxytestosterone formed enzymatically by incubation of [.C]-testosteron