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Titlebook: Chromosome Architecture; Methods and Protocol Mark C. Leake Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under

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1064-3745 expertsThis detailed new edition collects cutting-edge laboratory protocols, techniques, and applications in use by some of the leading international experts in the broad field of chromosome architecture. The book emphasizes the increasing physiological relevance of chromosome architecture investig
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https://doi.org/10.1007/978-981-15-7941-7omosome-associated activities. Here, we outline the technique of fluorescence recovery after photobleaching (FRAP) as a method to study the properties of YFP-tagged MukB in fluorescent foci. This method can provide important insight into the dynamics of MukB on DNA and be used to study its biochemical properties in vivo.
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Educational Writings in Chicago Yearse describe a method allowing the direct visualization of bacterial conjugation in live cells, including the fluorescent labeling of the conjugative pilus and the monitoring of plasmid DNA transfer from donor to recipient cells.
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Educational Writings in Columbia Yearsent three approaches which enable the mechanics to be probed under varying conditions. This includes fully adhered cells, initially adhered cells which lack an established cytoskeleton, and purified nuclei to study their isolated response.
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Young Dewey and Zeitgeist in Psychologyed method of protein copy number quantification directly in living cells. This enables quick and reliable estimations and comparison of the protein of interest abundance without implementing large-scale studies.
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Fluorescence Recovery After Photobleaching (FRAP) to Study Dynamics of the Structural Maintenance oomosome-associated activities. Here, we outline the technique of fluorescence recovery after photobleaching (FRAP) as a method to study the properties of YFP-tagged MukB in fluorescent foci. This method can provide important insight into the dynamics of MukB on DNA and be used to study its biochemical properties in vivo.
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