Titlebook: Chromosome Architecture; Methods and Protocol Mark C. Leake Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under

药物

Young Dewey and Zeitgeist in Psychologypy numbers is essential for revealing and better understanding of cellular behavior and functions. Here we describe a single-molecule fluorescence-based method of protein copy number quantification directly in living cells. This enables quick and reliable estimations and comparison of the protein of

Acquired

John Dewey and Progressive Educational processes on the molecular scale. These methods have also transformed the study of bacterial cell biology, which was previously obstructed by the limited spatial resolution of conventional microscopy. In the case of DNA-binding proteins, super-resolution microscopy can visualize the detailed spat

COLIC

Crohns-disease

https://doi.org/10.1007/978-981-15-7941-7 to determine structure and help regulate gene expression. Much of this mechanical perturbation is due to molecular machines such as topoisomerases which must stretch and twist DNA as part of various functions including DNA repair and replication. While the broad-scale mechanical response of nucleic

OTTER

https://doi.org/10.1007/978-3-319-93088-6o ensure their correct replication and segregation into progeny cells. Here we discuss techniques that can be used with live bacterial cells to analyze DNA replisome dynamics, double-strand chromosome breaks, and restart of repaired replication forks.

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Mark C. LeakeIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts