Titlebook: Chromosome Architecture; Methods and Protocol Mark C. Leake Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under

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书目名称Chromosome Architecture影响因子(影响力)




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书目名称Chromosome Architecture网络公开度




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书目名称Chromosome Architecture被引频次




书目名称Chromosome Architecture被引频次学科排名




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书目名称Chromosome Architecture年度引用学科排名




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书目名称Chromosome Architecture读者反馈学科排名

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Single-Molecule Narrow-Field Microscopy of Protein-DNA Binding Dynamics in Glucose Signal Transductused in bacteria. Here, we describe how these methods can be extended to larger eukaryotic, yeast cells, which contain subcellular compartments. We describe how to obtain single-molecule microscopy data but also how to analyze these data to track and obtain the stoichiometry of molecular complexes d

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Convolutional Neural Networks for Classifying Chromatin Morphology in Live-Cell Imaging,in machine learning have enabled researchers to automatically classify chromatin morphology in fluorescence microscopy images. In this protocol, we develop user-friendly tools to perform this task. We provide an open-source annotation tool, and a cloud-based computational framework to train and util

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Fluorescence Recovery After Photobleaching (FRAP) to Study Dynamics of the Structural Maintenance oescently tagged MukBEF forms distinct spots (or “foci”) composed of molecular assemblies in the cell, where it is thought to carry out most of its chromosome-associated activities. Here, we outline the technique of fluorescence recovery after photobleaching (FRAP) as a method to study the properties

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Atomic Force Microscopy of DNA and DNA-Protein Interactions,ications, one of its key advantages is its ability to visualize the substructure of single molecules and molecular complexes in an aqueous environment. Here, we describe the application of AFM to determine the secondary and tertiary structure of surface-bound DNA, and its interactions with proteins.

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Atomistic Molecular Dynamics Simulations of DNA in Complex 3D Arrangements for Comparison with Loweng detail at a resolution where experimental techniques cannot arrive. Molecular dynamics (MD) simulations of mechanically distorted DNA caused by agents like supercoiling and protein binding are computationally challenging due to the large size of the associated systems and timescales. However, now

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DNA-Protein Interactions Studied Directly Using iSCAT Imaging of GNP-Tagged Proteins,eatures are facile to include in a study. For single-molecule imaging this can be more difficult, because the constraints of the assay limit the variety of adducts that can be used. Surface-immobilized DNA provides an ideal compromise, and the use of interferometric scattering microscopy allows for