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Titlebook: Chromosome Architecture; Methods and Protocol Mark C. Leake Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under

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Chromosome Copy Number Measurement Using Flow Cytometry Analysis,cells and by detecting cell-scattered light their number and relative size can be measured as they pass through the beam. Labeling of molecules, usually via a fluorescent reporter, allows the amount of these molecules per cell to be quantified. DNA content can be estimated using this approach and he
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Visualizing the Replisome, Chromosome Breaks, and Replication Restart in ,o ensure their correct replication and segregation into progeny cells. Here we discuss techniques that can be used with live bacterial cells to analyze DNA replisome dynamics, double-strand chromosome breaks, and restart of repaired replication forks.
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High-Throughput Imaging of ,o cytologically analyze every mutant and screen for new genes involved in cell shape determination, cell division, and chromosome segregation. Here we describe a high-throughput method to image arrayed . mutant libraries using wide-field fluorescence microscopy. We provide a detailed description of
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https://doi.org/10.1007/978-981-15-7941-7ize a convolutional neural network to automatically classify chromatin morphology. Using cloud compute enables users without significant resources or computational experience to use a machine learning approach to analyze their own microscopy data.
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