Titlebook: Cell Cycle Control; Methods and Protocol Anna Castro,Benjamin Lacroix Book 2024 The Editor(s) (if applicable) and The Author(s), under excl

厨师

Mechanical Characterization of Murine Oocytes by Atomic Force Microscopy,es obtained by AFM are in agreement with the ones previously obtained by micropipette aspiration. Our protocol could help characterize the biophysical properties of oocytes or other types of large nonadherent samples in fundamental and medical research.

隐语

Measuring Mitotic Spindle and Microtubule Dynamics in Marine Embryos and Non-model Organisms,mes during cell division. Here, we describe a technique based on injection of purified proteins that enables the visualization of microtubules and chromosomes with a high contrast in several divergent marine embryos. We also provide analysis methods and tools to extract microtubule dynamics and moni

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Epithelium

跟随

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Whole-Mount Immunofluorescence Staining to Visualize Cell Cycle Progression in Mouse Oocyte Meiosiso visualize spindle morphology and microtubule attachments to kinetochores, using a technique we call “cold treatment,” at various phases of the meiotic cell cycle. This method allows the analysis of spindle structures at different meiosis I stages and at metaphase II. An adaptation of the protocol to the cell cycle stage of interest is described.

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构想

T. Hattori,H. Nohira,K. Takahashio visualize spindle morphology and microtubule attachments to kinetochores, using a technique we call “cold treatment,” at various phases of the meiotic cell cycle. This method allows the analysis of spindle structures at different meiosis I stages and at metaphase II. An adaptation of the protocol to the cell cycle stage of interest is described.

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Anna Castro,Benjamin LacroixIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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