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Titlebook: Cell Cycle Control; Methods and Protocol Anna Castro,Benjamin Lacroix Book 2024 The Editor(s) (if applicable) and The Author(s), under excl

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发表于 2025-3-21 17:10:51 | 显示全部楼层 |阅读模式
书目名称Cell Cycle Control
副标题Methods and Protocol
编辑Anna Castro,Benjamin Lacroix
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cell Cycle Control; Methods and Protocol Anna Castro,Benjamin Lacroix Book 2024 The Editor(s) (if applicable) and The Author(s), under excl
描述This detailed volume collects techniques to study the highly regulated cell cycle process. Beginning with chapters investigating these processes and assessing how cells respond when these complicated pathways are simplified by using synthetic biology and in vitro reconstitutions, the book continues by exploring how cells sense and respond to environmental conditions, different model systems and cellular types used to visualize cellular architecture during cell division, as well as innovative single cell microscopy techniques to highlight the heterogeneity of the cell population with respect to cell cycle progression. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Cell Cycle Control: Methods and Protocols. serves as an ideal guide for researchers attempting to elucidate this vital area of cell biology..
出版日期Book 2024
关键词Cell division; Synthetic biology; Environmental effects; In vitro reconstitution; Model systems; Single c
版次1
doihttps://doi.org/10.1007/978-1-0716-3557-5
isbn_softcover978-1-0716-3559-9
isbn_ebook978-1-0716-3557-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Studying Translesion DNA Synthesis Using , In Vitro Systems,esis, the DNA damage response, and genome integrity maintenance. We set out assays using . cell-free extracts to study translesion DNA synthesis (TLS), a branch of the DNA damage tolerance pathway that allows replication of damaged DNA. Using this system, we were able to recapitulate TLS activities
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,Cell Cycle–Specific Protein Phosphatase 1 (PP1) Substrates Identification Using Genetically Modifiees with a variety of regulatory subunits, and many of those are cell cycle regulated. Although several methods have been used to identify PP1 substrates, their cell cycle specificity is still an unmet need. Here, we present a new strategy to investigate PP1 substrates throughout the cell cycle using
发表于 2025-3-22 08:10:33 | 显示全部楼层
Dissecting the Multiple Functions of the Polo-Like Kinase 1 in the , Zygote,l functions, Plk1 also regulates cell polarization and cell fate during asymmetric cell divisions in . and .. Plk1 contains a specialized phosphoserine–threonine binding domain, the polo-box domain (PBD), which localizes and concentrates the kinase at its various sites of action within the cell in s
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Measuring Molecular Diffusion in Self-Organizing , Extracts by Fluorescence Correlation Spectroscopnction efficiently enough in a seemingly jammed environment remains elusive. Cell-free . extracts represent a powerful system for investigating the biochemistry and biophysics of living systems. Here we present a protocol for characterizing macromolecular diffusion in self-organizing cytoplasmic ext
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Manipulation of Embryonic Cleavage Geometry Using Magnetic Tweezers,nclear. In that context, new methods to mechanically manipulate these divisions are starting to emerge in different model systems. These are key to develop future innovative approaches and understand developmental mechanisms controlled by cleavage geometry. In particular, how cell cycle pace is regu
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Give and Take: The Reciprocal Control of Metabolism and Cell Cycle,ize, copies its . , prepares to divide, and divides. All these stages require a coordination of signaling pathways as well as adequate levels of energy and building blocks. These specific signaling and metabolic switches are tightly orchestrated in order for the cell cycle to occur properly. In this
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