Titlebook: Cell Cycle Control; Methods and Protocol Anna Castro,Benjamin Lacroix Book 2024 The Editor(s) (if applicable) and The Author(s), under excl

TRUST

固执点好

CAND

过多

https://doi.org/10.1007/978-94-017-2482-1dge live-cell sensors. These advanced single-cell imaging procedures provide promising opportunities for elucidating the molecular mechanisms underpinnings of heterogeneous responses in cell-cycle progression.

Pde5-Inhibitors

Exclaim

Artificial Modulation and Rewiring of Cell Cycle Progression Using Synthetic Circuits in Fission Yeeries of methods based on a minimal cell cycle module in the fission yeast . that allows for an unprecedented artificial control of cell cycle events, enabling the rewiring and remodeling of cell cycle progression.

英寸

IRATE

Investigating Heterogeneous Cell-Cycle Progression Using Single-Cell Imaging Approaches,dge live-cell sensors. These advanced single-cell imaging procedures provide promising opportunities for elucidating the molecular mechanisms underpinnings of heterogeneous responses in cell-cycle progression.

破布

Studying Translesion DNA Synthesis Using , In Vitro Systems,that occur naturally in vivo during early embryogenesis. This chapter describes protocols to detect chromatin-bound TLS factors by western blotting and immunofluorescence microscopy upon induction of DNA damage by UV irradiation, monitor TLS-dependent mutagenesis, and perform proteomic screening.

cipher

Measuring Molecular Diffusion in Self-Organizing , Extracts by Fluorescence Correlation Spectroscopracts using fluorescence correlation spectroscopy (FCS), which measures the motions on a distance scale of ~200 nm. The method can also be used to characterize diffusion in the cytoplasm as it progresses through different phases of the cell cycle.