Titlebook: Cell Cycle Control; Methods and Protocol Anna Castro,Benjamin Lacroix Book 2024 The Editor(s) (if applicable) and The Author(s), under excl

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Whole-Mount Immunofluorescence Staining to Visualize Cell Cycle Progression in Mouse Oocyte Meiosiso visualize spindle morphology and microtubule attachments to kinetochores, using a technique we call “cold treatment,” at various phases of the meiotic cell cycle. This method allows the analysis of spindle structures at different meiosis I stages and at metaphase II. An adaptation of the protocol

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Imaging and Analysis of Drosophila Neural Stem Cell Asymmetric Division,angement of the cytoskeleton, including microtubules and actin filaments. An additional level of complexity is present in asymmetric dividing stem cells because cytoskeleton elements are also regulated by polarity cues. The neural stem cell system of the fruit fly represents a simple model to dissec

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Cell Cycle Mapping Using Multiplexed Immunofluorescence, regulate cell cycle commitment and progression through DNA replication and cell division. These studies have also provided evidence of heterogeneity in cell cycle regulation among individual cells, even within a genetically identical population. . combines highly multiplexed imaging with manifold l

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Investigating Heterogeneous Cell-Cycle Progression Using Single-Cell Imaging Approaches,ogeneity, which often require synchronization procedures. However, recent advancements in cell-cycle sensors and single-cell imaging techniques have turned this heterogeneity into an advantage for investigating the molecular mechanisms underlying diverse responses. This has led to significant progre

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Book 2024ssessing how cells respond when these complicated pathways are simplified by using synthetic biology and in vitro reconstitutions, the book continues by exploring how cells sense and respond to environmental conditions, different model systems and cellular types used to visualize cellular architectu

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Foundations of Language Supplementary Serieshese processes are connected is still poorly understood. This chapter discusses basic concepts for a coordinated analysis of cell cycle progression and metabolism and provides specific protocols for studying these two connected processes in mammalian cells.