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Titlebook: Candida Albicans; Methods and Protocol Ronald L. Cihlar,Richard A. Calderone Book 2009 Humana Press 2009 DNA.Disease models of candidiaisis

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https://doi.org/10.1007/978-94-011-5030-9ncerns. In the present chapter, optimized conditions that promote biofilm formation within individual wells of microtiter plates are described. In addition, the method has proven useful in preparing . biofilms for investigation by a variety of microscopic and molecular techniques.
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Conditions for Optimal , Biofilm Development in Microtiter Platesncerns. In the present chapter, optimized conditions that promote biofilm formation within individual wells of microtiter plates are described. In addition, the method has proven useful in preparing . biofilms for investigation by a variety of microscopic and molecular techniques.
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A. Hieronimus-Leuba,P. Willekensy measuring the zone of growth inhibition that it produces on medium seeded with an indicator strain of . in standard bioassays. Additional procedures are outlined for determining the viabilities of drug-treated biofilms and for examining biofilm morphology by scanning electron microscopy.
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Detection and Quantitation of Antifungal SIgA Antibodies in Body Fluidsa are to be obtained. This chapter outlines methods for the measurement of anti-. SIgA antibodies in primary units and shows how to control the factors that influence antibody measurement in external secretions.
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Immunization Protocols for Use in Animal Models of Candidiasisect of this chapter. We focus on recent efforts with the Als family of cell wall proteins to serve as a model, and also offer immunization methods in candidiasis models that can be adapted to any antigen of the organism.
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