Titlebook: Candida Albicans; Methods and Protocol Ronald L. Cihlar,Richard A. Calderone Book 2009 Humana Press 2009 DNA.Disease models of candidiaisis

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SpringerBriefs in Space Developmenta result of their allogeneic transplant, cancer chemotherapy, or HIV infection. Consequently, immunization might be considered as an unlikely way to protect patients from such infection. Nonetheless, there are a number of investigations that indicate active immunization or the passive treatment with

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A. Hieronimus-Leuba,P. Willekensilm on a polycarbonate membrane filter, and capping it with a second, smaller membrane filter followed by a wetted paper disk of the type used in zone-of-inhibition assays. The entire assembly is transferred to agar medium containing the antifungal agent of interest. During subsequent incubation, th

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https://doi.org/10.1007/978-94-011-5030-9 device removal is often impossible, while in addition, resulting candidal infections are difficult to resolve due to their increased resistance to many antifungal agents. Susceptibility studies of clinical isolates are generally performed according to the CLSI standard, which measures planktonic ce

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A. Hieronimus-Leuba,P. Willekens ease of handling, technical feasibility, and experience with their use. In this chapter, we describe methods for two of the most popular murine models of disease caused by .. In an intravenously disseminated candidiasis (DC) model, immunocompetent mice are infected by lateral tail vein injections o

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R. S. Hornstein,J. K. Willoughby mice with . and subsequently harvesting blood and other tissue at specific time points during infection. The tissues are homogenized and the infecting . isolated. Finally, RNA is extracted from recovered . cells and subjected to microarray analysis.

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https://doi.org/10.1007/978-94-011-5030-9gically relevant protein and protein–nucleic acid complexes. Where complete (or nearly complete) genome sequence data are available for the organism from which the complexes are isolated, their components can be readily identified using mass spectrometry. The most widely used TAP-tag consists of a p