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Titlebook: Candida Albicans; Methods and Protocol Ronald L. Cihlar,Richard A. Calderone Book 2009 Humana Press 2009 DNA.Disease models of candidiaisis

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DNA Fingerprinting , Speciesthat drug resistance can be associated with strains from a particular clade and that strains can exhibit anatomical specificity. It may, therefore, be valuable in the near future to screen for strains with a propensity for drug resistance. While a number of methods exist for genetically fingerprinti
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Preparation of Samples for Proteomic Analysis of the , Cell Wallstgenomic era. Besides identification, the characterization of protein function is also a key objective of proteomic research. One of the major challenges for . biologists is how to interpret the large amounts of transcriptional analysis data, and assign function(s) to numerous uncharacterized genes
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Gene Expression in an , Infection Model mice with . and subsequently harvesting blood and other tissue at specific time points during infection. The tissues are homogenized and the infecting . isolated. Finally, RNA is extracted from recovered . cells and subjected to microarray analysis.
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https://doi.org/10.1007/978-3-211-85548-5 (1) preparation of monocytes from human PBMCs and (2) . differentiation of the monocytes into DCs by growth factors and cytokines. Cells obtained in this fashion are screened for the presence or absence of antigenic markers characteristic of DCs by flow cytometry.
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https://doi.org/10.1007/978-3-211-85548-5tic pathogen, . and its determinants of pathogenesis at these sites. The principal immunoglobulin isotype in mucosal secretions is secretory immunoglobulin A (SIgA). Unlike the circulatory system, mucosal surfaces are open systems in which the concentrations of immune factors are affected by diurnal
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The Endothelium As Target for Interventionsense against infections by . spp. include phagocytic cells, i.e., polymorphonuclear neutrophils (PMNs) and mononuclear phagocytes. A powerful . assay to assess host–pathogen interactions and study pathogenesis is the co-culture of phagocytic cells with a test fungus. The precise contribution of phag
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