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Titlebook: Candida Albicans; Methods and Protocol Ronald L. Cihlar,Richard A. Calderone Book 2009 Humana Press 2009 DNA.Disease models of candidiaisis

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书目名称Candida Albicans
副标题Methods and Protocol
编辑Ronald L. Cihlar,Richard A. Calderone
视频video
概述Provides current methods for experimentation with Candida albicans.Applies Immunological, Molecular, Genetic and Mircobiological approaches.Topics include evaluation of vaccines, virulence factor dete
丛书名称Methods in Molecular Biology
图书封面Titlebook: Candida Albicans; Methods and Protocol Ronald L. Cihlar,Richard A. Calderone Book 2009 Humana Press 2009 DNA.Disease models of candidiaisis
描述.Over the course of the past decade, there have been remarkable advances in the study of human pathogenic fungi. These developments have taken place throughout a wide range of disciplines, and have come as the result of newly available genome sequences of pathogens such as candida albicans and other model fungi. In Candida Albicans: Methods and Protocols, expert researchers explore these exciting new insights, focusing on the study of medically important fungi and Candida spp in particular. Chapters examine critical aspects of molecular methods, providing information on reporter gene assays, transformation, gene expression in vivo, and methods for large-scale gene disruption. At the same time, the work includes in-depth descriptions of disease models of candidiasis, facts about strain identification, and guidelines on the preparation of samples for proteomic investigations and tandem affinity purification. Composed in the highly successful "Methods in Molecular Biology™" series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls...Authoritative a
出版日期Book 2009
关键词DNA; Disease models of candidiaisis; FISH; Gene expression in vivo; Proteomics; Reporter gene assays; Toxi
版次1
doihttps://doi.org/10.1007/978-1-60327-151-6
isbn_softcover978-1-61737-761-7
isbn_ebook978-1-60327-151-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2009
The information of publication is updating

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Detection and Quantitation of Antifungal SIgA Antibodies in Body Fluidstic pathogen, . and its determinants of pathogenesis at these sites. The principal immunoglobulin isotype in mucosal secretions is secretory immunoglobulin A (SIgA). Unlike the circulatory system, mucosal surfaces are open systems in which the concentrations of immune factors are affected by diurnal
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Immunization Protocols for Use in Animal Models of Candidiasisa result of their allogeneic transplant, cancer chemotherapy, or HIV infection. Consequently, immunization might be considered as an unlikely way to protect patients from such infection. Nonetheless, there are a number of investigations that indicate active immunization or the passive treatment with
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Penetration of Antifungal Agents Through , Biofilmsilm on a polycarbonate membrane filter, and capping it with a second, smaller membrane filter followed by a wetted paper disk of the type used in zone-of-inhibition assays. The entire assembly is transferred to agar medium containing the antifungal agent of interest. During subsequent incubation, th
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Biofilm Analysis in the Artificial Throat Using FISHspecies. In order to investigate the interaction between different species within a biofilm, a reproducible, reliable model system has to be utilized and an appropriate system for species identification applied. The present chapter describes the artificial throat model, a model system for growing mi
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Animal Models of Candidiasis ease of handling, technical feasibility, and experience with their use. In this chapter, we describe methods for two of the most popular murine models of disease caused by .. In an intravenously disseminated candidiasis (DC) model, immunocompetent mice are infected by lateral tail vein injections o
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