书目名称 | Candida Albicans | 副标题 | Methods and Protocol | 编辑 | Ronald L. Cihlar,Richard A. Calderone | 视频video | | 概述 | Provides current methods for experimentation with Candida albicans.Applies Immunological, Molecular, Genetic and Mircobiological approaches.Topics include evaluation of vaccines, virulence factor dete | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .Over the course of the past decade, there have been remarkable advances in the study of human pathogenic fungi. These developments have taken place throughout a wide range of disciplines, and have come as the result of newly available genome sequences of pathogens such as candida albicans and other model fungi. In Candida Albicans: Methods and Protocols, expert researchers explore these exciting new insights, focusing on the study of medically important fungi and Candida spp in particular. Chapters examine critical aspects of molecular methods, providing information on reporter gene assays, transformation, gene expression in vivo, and methods for large-scale gene disruption. At the same time, the work includes in-depth descriptions of disease models of candidiasis, facts about strain identification, and guidelines on the preparation of samples for proteomic investigations and tandem affinity purification. Composed in the highly successful "Methods in Molecular Biology™" series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls...Authoritative a | 出版日期 | Book 2009 | 关键词 | DNA; Disease models of candidiaisis; FISH; Gene expression in vivo; Proteomics; Reporter gene assays; Toxi | 版次 | 1 | doi | https://doi.org/10.1007/978-1-60327-151-6 | isbn_softcover | 978-1-61737-761-7 | isbn_ebook | 978-1-60327-151-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Humana Press 2009 |
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