Titlebook: Candida Albicans; Methods and Protocol Ronald L. Cihlar,Richard A. Calderone Book 2009 Humana Press 2009 DNA.Disease models of candidiaisis

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Ronald L. Cihlar,Richard A. CalderoneProvides current methods for experimentation with Candida albicans.Applies Immunological, Molecular, Genetic and Mircobiological approaches.Topics include evaluation of vaccines, virulence factor dete

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https://doi.org/10.1007/978-3-211-85548-5 (1) preparation of monocytes from human PBMCs and (2) . differentiation of the monocytes into DCs by growth factors and cytokines. Cells obtained in this fashion are screened for the presence or absence of antigenic markers characteristic of DCs by flow cytometry.

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R. S. Hornstein,J. K. Willoughby mice with . and subsequently harvesting blood and other tissue at specific time points during infection. The tissues are homogenized and the infecting . isolated. Finally, RNA is extracted from recovered . cells and subjected to microarray analysis.

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A. Hieronimus-Leuba,P. Willekensxed species biofilms on shunt prostheses. The model is used in conjugation with fluorescent . hybridization (FISH), which facilitates identification and localization of the resident microorganisms within biofilms.

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Biofilm Analysis in the Artificial Throat Using FISHxed species biofilms on shunt prostheses. The model is used in conjugation with fluorescent . hybridization (FISH), which facilitates identification and localization of the resident microorganisms within biofilms.

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