Titlebook: Cancer Stem Cells; Methods and Protocol Federica Papaccio,Gianpaolo Papaccio Book 2024Latest edition The Editor(s) (if applicable) and The

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E. Y. Lasfargues,W. G. CoutinhoCSCs are rare, dormant cells and able to self-renew and maintain tumor development and heterogeneity. A new age of basic and clinical cancer research, reclassification of human tumors, and the development of novel therapeutic approaches will undoubtedly result from a better knowledge of CSCs. In ord

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E. R. Waelti,R. Kraft,H. Cottiertail CD44 detection by flow cytometry, a precise technique allowing to determine the percentage of positive cells and the mean fluorescent intensity reflecting the CD44 expression by cells in the samples. The protocol explained here can be used to detect CD44 from cell suspensions prepared from tiss

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New Frontiers in Mammary Pathology 1986ethods are used to detect and isolate CSCs by flow cytometry. Among these, measurement of aldehyde dehydrogenase (ALDH) activity within the cell is an assay widely used to identify and isolate CSCs from different types of solid tumors. The aldehyde dehydrogenase (ALDH) is a polymorphic enzyme respon

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Selling the idea — people in automationr types. Signaling from non-tumor cells in the tumor microenvironment (TME) enriches for and supports CSC. This complex cell–cell signaling in the heterogeneous TME presents a challenge for patient survival; however, it also presents an opportunity to develop new targeted therapies that can inhibit

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