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Titlebook: Cancer Stem Cells; Methods and Protocol Federica Papaccio,Gianpaolo Papaccio Book 2024Latest edition The Editor(s) (if applicable) and The

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书目名称Cancer Stem Cells
副标题Methods and Protocol
编辑Federica Papaccio,Gianpaolo Papaccio
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cancer Stem Cells; Methods and Protocol Federica Papaccio,Gianpaolo Papaccio Book 2024Latest edition The Editor(s) (if applicable) and The
描述This detailed new edition gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells. New chapters focus on the latest technologies that have improved our knowledge in this field, such as organoids, machine learning, nanoparticles, and other recent advances. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. .Authoritative and up-to-date, .Cancer Stem Cells: Methods and Protocols, Second Edition. provides researchers with the key techniques needed to increase our understanding of this important field of study..
出版日期Book 2024Latest edition
关键词Organoids; Machine learning; Nanoparticles; Intercellular interactions; CSCs
版次2
doihttps://doi.org/10.1007/978-1-0716-3730-2
isbn_softcover978-1-0716-3732-6
isbn_ebook978-1-0716-3730-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Isolating Cancer Stem Cells from Solid Tumors,CSCs are rare, dormant cells and able to self-renew and maintain tumor development and heterogeneity. A new age of basic and clinical cancer research, reclassification of human tumors, and the development of novel therapeutic approaches will undoubtedly result from a better knowledge of CSCs. In ord
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Surface Markers for the Identification of Cancer Stem Cells,these cells is challenging and is done mainly by detecting the expression of antigens specific to stem cells. Currently, there is a significant number of surface markers available which can detect cancer stem cells by directly targeting the specific antigens present in cells. These markers possess d
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CD44-Based Detection of CSCs: CD44 Immunodetection by Flow Cytometry,tail CD44 detection by flow cytometry, a precise technique allowing to determine the percentage of positive cells and the mean fluorescent intensity reflecting the CD44 expression by cells in the samples. The protocol explained here can be used to detect CD44 from cell suspensions prepared from tiss
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ALDH Activity Assay: A Method for Cancer Stem Cell (CSC) Identification and Isolation,ethods are used to detect and isolate CSCs by flow cytometry. Among these, measurement of aldehyde dehydrogenase (ALDH) activity within the cell is an assay widely used to identify and isolate CSCs from different types of solid tumors. The aldehyde dehydrogenase (ALDH) is a polymorphic enzyme respon
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