Titlebook: Cancer Stem Cells; Methods and Protocol Federica Papaccio,Gianpaolo Papaccio Book 2024Latest edition The Editor(s) (if applicable) and The

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Surface Markers for the Identification of Cancer Stem Cells,s, multiple analytical methods and techniques, including functional assays, cell sorting, filtration approaches, and xenotransplantation methods, are used to identify cancer stem cells. This chapter will overview the functional significance of cancer stem cells, their biological correlations, specific markers, and detection methods.

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In Vitro Tumorigenic Assay: A Tumor Sphere Assay for Cancer Stem Cells,m-free and nonadherent culture conditions. Here we provide a detailed methodology to generate and propagate spheres from isolated cell suspensions of tumor tissues and cell lines using a semisolid Matrigel.-based three-dimensional (3D) culture system.

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Methods to Study the Role of Mechanical Signals in the Induction of Cancer Stem Cells,ydrogel substrates of the desired stiffness, the isolation and culture of primary differentiated normal cells derived from the human mammary gland, and the assessment of their CSC attributes after oncogene-mediated transformation.

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