Titlebook: Cancer Stem Cells; Methods and Protocol Federica Papaccio,Gianpaolo Papaccio Book 2024Latest edition The Editor(s) (if applicable) and The

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Detection of Cancer Stem Cells in Normal and Dysplastic/Leukemic Human Blood,stages: a small number of pluripotent stem cells at the top, few lineage-restricted cells in the middle, and several terminally differentiated blood cells at the bottom. Although LSCs can differentiate into the hematopoietic lineage, they can also accumulate as immature progenitor cells, also known

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Co-Delivery Polymeric Poly(Lactic-Co-Glycolic Acid) (PLGA) Nanoparticles to Target Cancer Stem-Likeo consider the multifaceted tumor microenvironment (TME) that produce pro-tumoral niches, which are often resistant to chemo- and targeted therapies. In order to target the chemoresistant cancer stem-like cells (CSCs) and their supportive TME, in this chapter we describe a nanoparticle-based targete

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Generation of Cancer Stem Cells by Co-Culture Methods,mesenchymal stem cells (MSCs), adipocytes, cancer-associated fibroblasts (CAFs), endothelial cells, tumor-associated macrophages (TAMs), and other immune cells. These interactions occur through complex networks of cytokines, inflammatory factors, and several growth factors. Diverse techniques are em

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Deep Learning of Cancer Stem Cell Morphology,ing artificial intelligence (AI) require no previous expertise in image annotation. Herein, we describe the construction of AI models that recognize the CSC morphology in cultures and tumor tissues. The visualization of the AI deep learning process enables insight to be obtained regarding unrecogniz

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Multicellular Tumoroids for Investigating Cancer Stem-Like Cells in the Heterogeneous Tumor Microen cancer cells, as well as the other non-tumor cells using qPCR following sorting. The response to chemotherapeutic agents and expression of stem markers can be determined for cancer cells using flow cytometry, excluding the other cell types to get an accurate view of the cancer cells. Furthermore, t

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Prostate Cancer Organoids for Tumor Modeling and Drug Screening,personalized drug screening. Henceforth, PDOs hold great promise in precision medicine for cancer. Herein, we describe the detailed protocol to establish and propagate organoids derived from isolated cell suspensions of PCa patient tissues or cell lines using the 3D semisolid Matrigel™-based hanging