Titlebook: CCN Proteins; Methods and Protocol Masaharu Takigawa Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under exclusi

agglomerate

978-1-0716-2746-4The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

树胶

CCN Proteins978-1-0716-2744-0Series ISSN 1064-3745 Series E-ISSN 1940-6029

方舟

forebear

Intercept

Nachverhandlung von Outsourcing-Verträgenis family of intercellular signaling regulators, which are considered matricellular proteins and were once referred to as “signal conductors” but have recently been renamed “Cellular Communication Network Factors.” Their repertoire of functions beyond their role as matricellular proteins is also des

CRACK

Pepsin

https://doi.org/10.1007/978-3-8350-9318-8ein-protein binding of endogenously expressed proteins. To confirm binding between CCN2 and Rab14 GTPase (Rab14) in chondrocytes, we performed a PLA using chondrocytic HCS-2/8 cells. The protocol in this chapter introduces an optimized technique for visualizing intracellular interactions of CCN2 and

灯丝

https://doi.org/10.1007/978-3-8350-9318-8d healing, and cancer. CCN factors and fragments generated by metalloproteinases-dependent cleavage are often associated with extracellular matrix (ECM) or small extracellular vesicles (sEVs) such as exosomes or matrix-coated vesicles. We provide reliable methods and protocols for Western blotting t

CIS

https://doi.org/10.1007/978-3-8350-9318-8 (EVs) such as exosomes or matrix-coated vesicles. CCN factors and fragments loaded on/in EVs may play key roles in cell communication networks in cancer biology, bone and cartilage metabolism, wound healing, and tissue regeneration. CCN proteins and EVs/exosomes are found in body fluids, such as bl

obtuse

https://doi.org/10.1007/978-3-8350-9318-8of CCN proteins, the analysis of the binding partners is the first step toward understanding the mechanisms of actions of CCN proteins. This chapter describes two approaches used for such analyses: a solid-phase binding assay, which is suitable for confirming the binding easily because of its simpli