Titlebook: CCN Proteins; Methods and Protocol Masaharu Takigawa Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under exclusi

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Transfection, Spinfection, Exofection, and Luciferase Assays for Analysis of CCN Genes Expression Me or the transcriptional activity of matrix metalloproteinase 3 (MMP3). We discovered that MMP3 in exosomes is transferable to recipient cells and then translocates into cell nuclei to transactivate the . gene. Exosomes and liposomes enable molecular transfection to recipient cells in vitro and in vi

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Protocol for CRISPR/Cas Genome Editing for Investigating Cell Communication NetworkISP3. The second member, CCN2/CTGF is a matricellular protein that promotes extracellular matrix (ECM) synthesis and controls angiogenesis. On the other hand, moonlighting/matrix metalloproteinase 3 (MMP3) is an ECM-degrading enzyme that also functions as an intracellular transcription factor. Impor

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Evaluation of the Effects of CCN4 on Pancreatic Beta Cell Proliferationappearance of diabetes. Using antibody arrays, we identified CCN4/Wisp1 as a circulating factor enriched in preweaning mice, a period in which beta cells exhibit a dramatic increase in number. This finding led us to investigate the involvement of CCN4 in beta cell proliferation. We demonstrated that

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Analysis of CCN Functions in Liver Regeneration After Partial Hepatectomyly to restore the original liver mass through a combination of hepatocyte proliferation and hypertrophy to maintain homeostasis. Experimentally, 2/3 partial hepatectomy in mice has been used extensively as a model to dissect the molecular mechanism of liver regeneration and the genetic networks invo

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Analysis of Chemotactic Property of CCN2/CTGF in Intramembranous Osteogenesising, and fracture healing of bones are advanced through intramembranous osteogenesis. Chemotaxis of preosteoblasts toward future bone formation sites observed in the early stage of intramembranous osteogenesis is a critical cellular process for normal bone formation. However, molecular biological me

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