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Titlebook: CCN Proteins; Methods and Protocol Masaharu Takigawa Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under exclusi

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Evaluation of the Effects of CCN4 on Pancreatic Beta Cell Proliferation CCN4 promotes adult beta cell proliferation in vitro in cultured isolated islets, and in vivo in islets transplanted into the anterior chamber of the eye. In this chapter, we present the methodology that was used to study proliferation in both settings.
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CCN Proteins (Cellular Communication Network Factors): Expanding Their Repertoire Toward a New Concis family of intercellular signaling regulators, which are considered matricellular proteins and were once referred to as “signal conductors” but have recently been renamed “Cellular Communication Network Factors.” Their repertoire of functions beyond their role as matricellular proteins is also des
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Western Blot Protocols for Analysis of CCN Proteins and Fragments in Exosomes, Vesicle-Free Fractiond healing, and cancer. CCN factors and fragments generated by metalloproteinases-dependent cleavage are often associated with extracellular matrix (ECM) or small extracellular vesicles (sEVs) such as exosomes or matrix-coated vesicles. We provide reliable methods and protocols for Western blotting t
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Evaluation of the Molecular Interaction Between CCN Protein and Its Binding Partners: A Solid-Phase of CCN proteins, the analysis of the binding partners is the first step toward understanding the mechanisms of actions of CCN proteins. This chapter describes two approaches used for such analyses: a solid-phase binding assay, which is suitable for confirming the binding easily because of its simpli
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