Titlebook: CCN Proteins; Methods and Protocol Masaharu Takigawa Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under exclusi

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https://doi.org/10.1007/978-3-8350-9318-8M) or small extracellular vesicles (sEVs) such as exosomes or matrix-coated vesicles. We provide reliable methods and protocols for Western blotting to analyze CCN factors and fragments in cells, sEVs, and vesicle-free fractions.

娘娘腔

rectum

https://doi.org/10.1007/978-3-662-25294-9artial hepatectomy in mice has been used extensively as a model to dissect the molecular mechanism of liver regeneration and the genetic networks involved. Herein, we describe the protocols for partial hepatectomy and analyses of pertinent CCN protein functions.

Outmoded

Western Blot Protocols for Analysis of CCN Proteins and Fragments in Exosomes, Vesicle-Free FractionM) or small extracellular vesicles (sEVs) such as exosomes or matrix-coated vesicles. We provide reliable methods and protocols for Western blotting to analyze CCN factors and fragments in cells, sEVs, and vesicle-free fractions.

Instinctive

Evaluation of the Molecular Interaction Between CCN Protein and Its Binding Partners: A Solid-Phase escribes two approaches used for such analyses: a solid-phase binding assay, which is suitable for confirming the binding easily because of its simplicity and cost advantage, and a surface plasmon resonance assay, which can determine the binding affinities between CCN proteins and their partners.

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冷漠

鉴赏家

https://doi.org/10.1007/978-3-8350-9318-8r polymer-based precipitation, affinity purification, ultracentrifugation methods combined with the ultrafiltration method for isolating CCN-loaded exosomes/EVs from 2D and 3D cultured tissues, and proteome analysis using mass spectrometry for comprehensive analysis of CCN proteins.

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Cougar