Titlebook: CCN Proteins; Methods and Protocol Masaharu Takigawa Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under exclusi

迅速成长

https://doi.org/10.1007/978-3-663-19744-7formation through intramembranous osteogenesis. In this chapter, we describe in detail the procedures of the in vivo and in vitro assays to investigate the chemotactic property of CCN2/CTGF and its underlying molecular biological mechanisms during intramembranous osteogenesis.

Seizure

Comprehensive Method for Exosome Isolation and Proteome Analysis for Detection of CCN Factors in/on r polymer-based precipitation, affinity purification, ultracentrifugation methods combined with the ultrafiltration method for isolating CCN-loaded exosomes/EVs from 2D and 3D cultured tissues, and proteome analysis using mass spectrometry for comprehensive analysis of CCN proteins.

BLAND

anthropologist

Protocol for CRISPR/Cas Genome Editing for Investigating Cell Communication Networksing the CRISPR/Cas9 system, demonstrating the sequential regulatory events of the MMP3-CCN2 axis in the microenvironment. Notably, our protocol is useful for generation of CCN knockout cells as well. Here we serve a protocol of the CRISPR/Cas9-based gene targeting in cultured cells for investigating cellular communication network.

ZEST

冰雹

类似思想

ITCH

Goblet-Cells

Conserve

CCN Proteins (Cellular Communication Network Factors): Expanding Their Repertoire Toward a New Conccribed to aid in future studies. Advanced research concerning their relevance to pathology is briefly introduced as well. The information provided in this chapter is expected to be useful for readers of subsequent chapters.