Titlebook: Bioluminescence Methods and Protocols; Robert A. LaRossa Book 19981st edition Springer Science+Business Media New York 1998

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Luminometric Measurement of Malate and Glucose-6-Phosphate in Mammalian Tissues (., e.g., .. .), only nanomolar or subnanomolar quantities can be expected to be present in small samples obtained from isolated cells or cell cultures. Such quantities are at best barely detectable by conventional spectrophotometric or fluorimetric procedures

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Visualization of Bioluminescenceist of different luminometers and cameras that are available. These instruments have been used to detect bioluminescence in a number of organisms using either bacterial luciferase (.; ., .) or firefly luciferase (.; ., .) as reporters. Sensitivity of the newer luminometers ranges from six to eight l

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Microscopic Imagery of Mammalian Cells Expressing an Enhanced Green Fluorescent Protein Gonen, cell transformation, and cell lineage in VIVO and in real time. Unlike other bioluminescent reporters, the chromophore in GFP is mtrinsic to the primary structure of the protein, and GFP does not require additional factors other than molecular oxygen (..) to fluoresce (., .). GFP emits bright gre